Biosynthesis of Silver Nanoparticles using Ajuga iva leaf extract and evaluating their antibacterial activity against E. coli and Streptococcus Bacteria (2025)

Green Synthesis of Silver Nanoparticles using Ajuga parviflora Benth and Digera muricata Leaf extract: Their Characterization and Antimicrobial Activity

Abdur Rab

Revista De Chimie, 2020

The aim of the present study is to search out nontoxic silver nanoparticles synthesized from the leaf extract of two plants Ajuga parviflora Benth and Digera muricata for antimicrobial activity. The plants used in this investigation are rich in alkaloids, flavonoids, steroids, terpenoids, protein, amino acids, carbohydrate, quninones, phenols and tannins. The formation of nanoparticles were confirmed by UV/Visible spectroscopy, peaks at 423nm for Ajuga parviflora Benth and 408nm for Digera muricata. The morphology of the silver nanoparticles was established through state of the art spectroscopic tools. SEM analysis reveals average size of AgNPs 18 nm for Digera muricata and 22 nm for Ajuga parviflora Benth respectively while transmission electron microscopy confirms that AgNPs are spherical in shape. The synthesized nanoparticles were subjected to Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Pseudomonas aeruginosa. The results suggest that the silver nanoparti...

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Plant Based Green Synthesis, Characterization and Antimicrobial Investigation of Silver Nanoparticles from Khaya senegalensis Aqueous Leaf Extract

Mela Yoro

International Journal of Scientific Research in Chemistry(IJSRCH)

In this research article, Silver nanoparticles were synthesized from Khaya senegalensis leaf extracts via green route. Spectroscopic study revealed color change of the solution from yellow to light brown within 25 min of addition of the AgNO3 against leaf aqueous extract with constant stirring. Beyond this time, no further change in color after the nucleation of the metal ions indicating that phytoconstituents of Khaya senegalensis resulted in the reduction of Ag+ to Ag0, a phenomenon that could be attributed to the surface Plasmon excitement of AgNPs. The bio fabricated silver nanoparticles were characterized using UV – Visible, FTIR and RXD so as to be certain of its formation before being deployed in the antimicrobial studies. The UV-Vis spectral analysis of the AgNPs from the leaf extract showed maximum absorbance of 2.01AU at a corresponding wavelength (λ max) of 500nm. The X-Ray Diffraction patterns of green synthesized AgNPs showed that the structure of the nanoparticles under research have face centered cubic (fcc) and spinel like structures with biosynthesized AgNPs having the average particle size of 65.5nm. The antimicrobial studies of Silver nanoparticles were conducted against B. subtilis, K. pneumonia (gram +ve bacteria) and two fungi, A. niger and C. albicans. Different concentrations of 100, 200, 300, 400 and 500μg/L of Silver nanoparticles were tested against each pathogen. The inhibition zone increases generally with increase in concentrations of silver nanoparticles. At higher concentration of 500μg/L, the zones of inhibition were in the following order; 18.5mm, 26.3mm, 23.5mm, and 24.4mm for Bacillus subtilis, Klebsiella pneumonia, Aspergillus niger and Candida albicans respectively. For each concentration investigated, C. albicans, demonstrated higher zone of inhibition as opposed to all other pathogens under investigation. The results of this research therefore indicated that Silver Nanoparticles synthesized from Khaya senegalensis plant extracts demonstrated potent antimicrobial activity on the selected pathogenic microbes, hence be used as antimicrobial agent against the organisms in question.

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Preparation and Evaluation of Silver nanoparticles by using Plant Extracts for Antimicrobial Activity

Avi Dubey

2021

Nanotechnology is the modern research field which deals with design, synthesis and manipulation of particles structure range from 1-100nm in dimension. A nanoparticle is ultra-fine unit with dimension less than 100 nm (1 nm=10-9 meter).Because of their small size they have unique characteristics and their applications in various field such as medicines, engineering and environmental remediation. Nanoparticles can be prepared by various aapproaches like physical, chemical and biological .The biological approach is the emerging approach because it is easier than other methods. In this green synthesis aqueous solution of extract of Azadirathcha indica and AgNo3 were taken for study. The nanoparticles were prepared by using plant extract and silver metal ions. The nanoparticles were characterized by different spectral methods like UV, IR, DLS, SEM, XRD and Zeta analysis.

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SYNTHESIS OF PLANT-MEDIATED SILVER NANOPARTICLES USING AERVA LANATA LEAF AQUEOUS EXTRACT AND EVALUATION OF ITS ANTI BACTERIAL ACTIVITIES

Santhosh Sigamani

In the present study the synthesis of silver nanoparticles has been carried out from the Aerva lanata aqueous Leaf extract. The reduction of silver nitrate led to the formation of Silvernanoparticles within one day at room temperature. The size, shape and elemental analysis were carried out using TEM with EDX, FT-IR and visible absorption spectroscopy. The prepared AgNPs showed surface Plasmon resonance center at 425 nm with average particle size of 15-20 nm. Crystalline nature of the nanoparticles is confirmed from the XRD patterns. From the FTIR spectra it is found that the biomolecules are responsible for capping in silver nanoparticles. The synthesized silver nanoparticles have been investigated against human pathogenic bacteria.

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Synthesis of Biogenic Silver Nanoparticles From Medicinal Plant And It’s Antibacterial Activity

Hiral Vaghela

IOSR Journal of Applied Chemistry, 2016

Biological synthesis of nanoparticles from medicinal plant is gaining importance due to its ecofriendliness. The synthesis of metal nanoparticles is a broad research area due to the potential applications for the development of different novel technologies. In our research work, we have described a cost effective and eco-friendly technique for the green synthesis of silver nanoparticles and evaluated their Antibacterial activity. Synthesis and characterization of silver nanoparticles were carried out by using Crateva religiosa plant bark extract as reducing agent as well as capping agent. The Synthesized nanoparticles were characterized with UV-Visible spectrometry(UV-Vis), Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM) and X-ray Diffraction Spectroscopy(XRD). The antibacterial activity of silver nanoparticles has been Observed.

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Synthesis and Characterization of Bioinspired Silver Nanoparticles by Aqueous Leaf Extract of Indigofera cassioides: Evaluation of Antimicrobial and Cytotoxic Activity

abdul lathiff

Journal of nanoscience and technology, 2019

The green synthesis of silver nanoparticles (AgNPs) has increasingly promising due to their potential applications in nanomedicine. For the first time, silver nanoparticles (AgNPs) were synthesized from aqueous leaf extract of Indigofera cassioides. The AgNPs were characterized by UV-Vis spectroscopy and the characteristic surface plasmon resonance peak was observed at 414 nm. The Fourier transform infrared spectroscopy (FT-IR) revealed the phytochemicals in the plant extract which assisted bioreduction, capping and stabilizing agent. Morphology of AgNPs was characterized by transmission electron microscopy (TEM) and they were spherical shape with average particle size as 20 nm. The face centered cubic (FCC) crystalline structure of the AGNPs was confirmed by X-ray diffraction. Dynamic light scattering (DLS) and zeta potential analysis revealed that the average hydrodynamic diameter of the AGNPs as 80 nm and the negative zeta potential value of-36.6 mV indicates good stability. Antimicrobial studies disclosed that the biosynthesized AgNPs possess strong activity against pathogenic bacteria. The cytotoxicity study is concealed that the AgNPs exhibit selective cytotoxicity against the tested cell lines and a pronounced activity was observed against human breast cancer cells (MCF-7). The present study concludes that for the biosynthesized AgNPs could be used as an antimicrobial and anticancer agent.

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Review green synthesis of silver nanoparticles by using plant extracts and their antimicrobial activity

Dr. Ahmed El-Shabasy, Abdullah Mashraqi, Mohamed Al Abboud

Interest in the biosynthesis of nanoparticles has increased in the last era by researchers. Nanoparticles have several applications in different fields like optoelectronics, magnetic devices, drug delivery, and sensors. Nanoparticle synthesis by green methods is safe for the environment and should be explored and encouraged popularly since various plants' have the high extent to form these nanoparticles. Worldwide, UV spectroscopy, Xray diffraction, Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), Dynamic Light Scattering (DLS), Atomic Force Microscopy (AFM) besides Fourier Transform Infrared Spectroscopy (FTIR) are used in many ways for characterize nanoparticles. The most advantageous use of AgNPs is their great attribution to be used as antimicrobial agents. Finally, concept of AgNPs synthesis is deserved to be the modern technical and medical concern. The current review shows a complete comprehensive and analytical survey of the biosynthesis of AgNPs with a particular focus on their activities as antimicrobials and the possible theories of their effect on the microbial cell and all influenced secondary metabolites.

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Synthesis of silver nanoparticles using plants extract and analysis of their antimicrobial property

Jayanthi Abraham

Journal of Saudi Chemical Society, 2012

Plants extract from Ocimum tenuiflorum, Solanum tricobatum, Syzygium cumini, Centella asiatica and Citrus sinensis was used for the synthesis of silver nanoparticles (Ag NPs) from silver nitrate solution. Ag NPs were characterized by UV-vis spectrophotometer, X-ray diffractometer (XRD), atomic force microscope (AFM) and scanning electron microscope (SEM). The formation and stability of the reduced silver nanoparticles in the colloidal solution were monitored by UV-vis spectrophotometer analysis. The mean particle diameter of silver nanoparticles was calculated from the XRD pattern according to the line width of the plane, refraction peak using the Scherrer's equation. AFM showed the formation of silver nanoparticle with an average size of 28 nm, 26.5 nm, 65 nm, 22.3 nm and 28.4 nm corresponding to O. tenuiflorum, S. cumini, C. sinensis, S. tricobatum and C. asiatica, respectively. SEM determination of the brown color stable samples showed the formation of silver nanoparticles and well dispersed nanoparticles could be seen in the samples treated with silver nitrate. Antimicrobial activity of the silver bio-nanoparticles was performed by well diffusion method against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae. The highest antimicrobial activity of silver nanoparticles synthesized by S. tricobatum, O. tenuiflorum extracts was found against S. aureus (30 mm) and E. coli (30 mm) respectively. The Ag NPs synthesized in this process has the efficient antimicrobial activity against pathogenic bacteria. Of these, silver nanoparticles are playing a major role in the field of nanotechnology and nanomedicine.

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Biosynthesis and Characterisation of Silver Nanoparticles using Leaf Extract of Achras sapota l. for its Antimicrobial Activity

International Journal of Scientific Research in Science and Technology IJSRST

International Journal of Scientific Research in Science and Technology, 2020

Nanotechnology is a field that is rapidly growing, making an impact in all spheres of human life. In the current study, silver nanoparticles were synthesized using the ethanolic leaf extract of Achras sapota. Characterization was carried out using UV-Visible spectroscopy, FTIR, XRD, SEM and TEM. The formation of AgNPs was confirmed through UV-Visible spectroscopy by the colour change. Based on the XRD pattern, the crystalline property of the AgNPs was established. The functional group present in the ethanolic leaf extract of Achras sapota is responsible for the reduction of the Ag+ ion which was studied through FTIR. From the SEM and TEM analysis, it was found that the formed nanoparticles are spherical in shape and nano in size. The biosynthesized AgNPs was evaluated for its antimicrobial activity against gram positive bacteria (S. aureus and L. bacillus) and gram negative bacteria (E. coli and P. aeruginosa) using disc diffusion for preliminary screening of antimicrobial activity and dilution method for evaluation of antibacterial effectiveness and effect of silver nanoparticles on bacterial growth and it was found to exhibit potential antimicrobial activity. The biosynthesized AgNPs was found to be efficient in terms of reaction time as well as stability, eco-friendly and cost effective.

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Synthesis And Characterization Of Silver Nanoparticles Using Flower Extract Ofaerva Lanata And Evaluation Of Its antimicrobial Activities

Dr. J. THOMAS JOSEPH PRAKASH j

2020

Silver nanoparticles (AgNPs) with flower extract of Aerva lanata are successful in the process of synthesis. The characterization of obtained AgNPs are accomplished by the following methods like UV-Visible Spectrometer, Field Emission Scanning Electron Microscopy paired with Energy Dispersive Xray Spectroscopy, X-ray Diffraction, Fourier Transform Infrared Spectroscopy, Dynamic Light Scattering. The spherical morphologies are confirmed, with the size of range 10 to 50 nm. The Energy Dispersive X-ray Spectroscopy present in the elemental absorption peak at 3 KeV. The existence of elemental silver in the sample is also proved by the planes (111), ( 200), (220) and (311) corresponds peaks in the X-Ray Diffraction patterns which implies the crystalline nature of the nanoparticles in the face centered cubic structure. The evaluation of size dis tribution of nanoparticles were done using Dynamic Light Scattering measurements. Additionally, nanoparticles show effective antibacterial and an...

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Biosynthesis of Silver Nanoparticles using Ajuga iva leaf extract and evaluating their antibacterial activity against E. coli and Streptococcus Bacteria (2025)
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